Transcriptomics

Dataset Information

5

Gene expression profiles of CD4 T cells and CD45+ HLA-DR+ cells during experimental allergic rhinitis in humans.


ABSTRACT: Six patients with seasonal allergic rhinitis were challenged daily for 8 days with birch pollen extract. A mucosal biopsy was obtained from one nostril at basline (day 0) and from the other nostril after allergen challenge (day 9). The mucosal biopsies were digested into single cells, and then sorted into CD4 T cells and CD45+HLA-DR+ cells. Total RNA was extracted, amplified using whole transcriptome amplification, and gene expression was profiled on microarrays. The study design consisted of 6 subjects, 2 cell types (CD4 T cells, CD45+ HLA-DR+ cells), and 2 conditions (baseline, allergen challenge).

ORGANISM(S): Homo sapiens  

SUBMITTER: Patrick Holt   Ralph Dollner  Anthony Bosco  Frode Jahnsen 

PROVIDER: E-GEOD-49782 | ArrayExpress | 2015-09-01

SECONDARY ACCESSION(S): GSE49782PRJNA214886

REPOSITORIES: GEO, ArrayExpress

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