Transcriptomics

Dataset Information

37

IL-2-dependent gene expression by human regulatory T cells


ABSTRACT: This study determined the genes that are differetially expressed when regulatory T cells were stimulated in vitro with IL-2 388 Affymetrix targets were found to be up or down regulated in human regulatory T cells by IL-2 by two-fold or more. Gene enrichment analyss identified that IL-2 functions to regulate gene expression for mRNAs important for immune system processes, cell growth, cell death, inflammatory response, cell migration, and the Jak/Stat pathway, among others. A subset of these differentially expressed genes were distinctively expressed between individual subjects. Human regulatory T cells (Tregs) cells were FACS purified based on expression of CD4+ CD127-lo CD25-HI. These cells were cultured for 24 hr in media with or without IL-2 (100 u/ml). The cells were harvested, total RNA was prepared and processed to probe Affymetrix human Gene ST1.0 arrays.

ORGANISM(S): Homo sapiens  

SUBMITTER: Thomas R Malek  Alberto Pugliese    

PROVIDER: E-GEOD-49817 | ArrayExpress | 2015-08-19

SECONDARY ACCESSION(S): GSE49817PRJNA214999

REPOSITORIES: GEO, ArrayExpress

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Publications

Selective IL-2 responsiveness of regulatory T cells through multiple intrinsic mechanisms supports the use of low-dose IL-2 therapy in type 1 diabetes.

Yu Aixin A   Snowhite Isaac I   Vendrame Francesco F   Rosenzwajg Michelle M   Klatzmann David D   Pugliese Alberto A   Malek Thomas R TR  

Diabetes 20150109 6


Low-dose interleukin-2 (IL-2) inhibited unwanted immune responses in several clinical settings and is currently being tested in patients with type 1 diabetes (T1D). Low-dose IL-2 selectively targets regulatory T cells (Tregs), but the mechanisms underlying this selectivity are poorly understood. We show that IL-2-dependent STAT5 activation in Tregs from healthy individuals and patients with T1D occurred at an ∼10-fold lower concentration of IL-2 than that required by T memory (TM) cells or by in  ...[more]

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