Transcriptomics

Dataset Information

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Identification of Smaug target mRNAs in the early Drosophila embryo using RIP-Chip


ABSTRACT: To identify Smaug’s target mRNAs on a genome-wide scale we used ribonucleoprotein (RNP) co-immunoprecipitation followed by microarray analysis of the co-purifying mRNAs (RIP-Chip). Extracts, prepared from wild-type embryos collected 0-3 hours post-egglaying, were immunoprecipitated with an anti-Smaug antibody (Smaug RIPs) while control immunoprecipitations using non-immune serum served as a negative control (control RIPs). Genome-wide transcript expression in wild-type embryos were also assessed and used as reference. There are 11 array experiments presented here: 1. gene expression profiling of total mRNA sample extracted from wild-type 0-3 hour embryos (3 technical replicates performed using a pooled input reference sample); 2. RNA co-immunoprecipitations of endogenous Smaug (Smaug RIPs) from wild-type 0-3 hour embryos (3 biological replicates and 1 technical replicates); 3. control RNA co-immunoprecipitations (control RIPs) from wild-type 0-3 hour embryos (3 biological replicates and 1 technical replicates).

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Craig A Smibert   Zhiyong Yang  Najeeb U Siddiqui  J T Westwood  Linan Chen  Xiao Li  Quaid D Morris  Jason G Dumelie  John D Laver  Matthew H Cheng  Howard D Lipshitz 

PROVIDER: E-GEOD-49943 | ArrayExpress | 2013-12-03

SECONDARY ACCESSION(S): GSE49943PRJNA215420

REPOSITORIES: GEO, ArrayExpress

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