Transcriptomics

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6

Resveratrol improves adipose insulin signaling and reduces the inflammatory response in adipose tissue of rhesus monkeys on a high-fat, high-sugar diet.


ABSTRACT: Obesity is associated with a chronic, low-grade, systemic inflammation that may contribute to the development of insulin resistance and type 2 diabetes. Resveratrol, a natural compound with anti-inflammatory properties, is shown to improve glucose tolerance and insulin sensitivity in obese mice and humans. Here we tested the effect of a 2-year resveratrol administration on the pro-inflammatory profile and insulin resistance caused by a high-fat, high-sugar (HFS) diet in white adipose tissue (WAT) from rhesus monkeys. Eighty mg/day of resveratrol for 12-month followed by 480 mg/day for the second year decreased adipocyte size, increased sirtuin 1 expression, decreased NF-kB activation and improved insulin sensitivity in visceral but not subcutaneous WAT from HFS-fed animals. These effects were reproduced in 3T3-L1 adipocytes cultured in media supplemented with serum from monkeys fed HFS +/- resveratrol diets. In conclusion, chronic administration of resveratrol exerts beneficial metabolic and inflammatory adaptations in visceral WAT from diet-induced obese monkeys. Twenty-four adult (7-13 years old) male rhesus monkeys (Macaca mulatta) were housed individually in standard nonhuman primate caging on a 12h light/12h dark cycle, room temperature (78 +/- 2 degrees F), and humidity at 60 +/- 20%. One pairing was maintained throughout the study; all other monkeys had extensive visual, auditory, and olfactory but limited tactile contact with monkeys housed in the same room. Monkeys received 2 meals per day at estimated ad libitum levels throughout the study. Water was always available ad libitum. Monkeys were monitored minimally 3 times daily by trained animal care staff. During baseline assessments, all monkeys were maintained on a commercially available closed formula monkey chow. After baseline assessment, four male rhesus monkeys remained on the healthy standard diet (SD), 10 male rhesus monkeys were begun on a high fat/high sucrose (HFS) diet and 10 male rhesus monkeys were begun on a high fat/high sucrose (HFS) diet plus Resveratrol, 80mg/day. After one year of dietary intervention, the amount of resveratrol was increased to 480mg/day for one additional year. Tissues were then harvested for the array experiments.

ORGANISM(S): Macaca mulatta  

SUBMITTER: Kevin G Becker   Michel Bernier  Luigi Ferrucci  Theresa M Ward  Jonathan S Bogan  Yolanda Jimenez-Gomez  Joseph A Baur  Rafael de Cabo  Maria M Malagon  Edward M Tilmont  Joanne S Allard  Julie A Mattison  Ruin Moaddel  Richard L Herbert  Jonathan P Belman  Dan L Longo  Placido Navas  Caitlin M Younts  Mitesh Sanghvi  Christopher H Morrell  Kaitlyn Lewis  Kevin J Pearson  Alex M Sossong  Alejandro Martin-Montalvo  Hector H Palacios  Josephine M Egan 

PROVIDER: E-GEOD-50005 | ArrayExpress | 2014-04-21

SECONDARY ACCESSION(S): GSE50005PRJNA215790

REPOSITORIES: GEO, ArrayExpress

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