Proteomics

Dataset Information

0

Polyubiquitination analysis upon GSK3 inhibition in HeLa cell extracts


ABSTRACT: HeLa cell extracts with or without GSK3 enzyme inhibition were assayed using protein microarrays in order to detect GSK3-dependent changes in protein polyubiquitination. HeLa lysates in triplicates were supplemented with ubiquitin and incubated on protein microarrays (ProtoArray 5.0; Invitrogen) in the presence or absence of the GSK3 inhibitor SB-216763. Polyubiquitination of the arrayed proteins was detected using specific antibodies. ProtoArray 5.0 contains over 9,000 full-length human proteins purified and arrayed in duplicate under native conditions to maximize functionality.

ORGANISM(S): Homo sapiens  

SUBMITTER: Emil Karaulanov  Christof Niehrs   Sergio P Acebron    

PROVIDER: E-GEOD-50629 | ArrayExpress | 2014-05-15

SECONDARY ACCESSION(S): GSE50629PRJNA218117

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-07-30 | E-GEOD-50866 | ArrayExpress
2012-07-13 | E-GEOD-30496 | ArrayExpress
| PRJNA218117 | ENA
2010-06-19 | E-GEOD-19841 | ArrayExpress
2012-03-25 | E-GEOD-35171 | ArrayExpress
2010-05-26 | E-GEOD-10452 | ArrayExpress
2013-07-03 | E-GEOD-48478 | ArrayExpress
2015-07-27 | E-GEOD-51043 | ExpressionAtlas
2018-12-30 | E-MTAB-5985 | ArrayExpress
2008-02-13 | GSE10452 | GEO