Genomics

Dataset Information

358

DNA Topoisomerase 1α Promotes RNA-directed DNA Methylation and Histone Lysine 9 Dimethylation at Transposable Elements in Arabidopsis [Bisulfite-Seq]


ABSTRACT: RNA-directed DNA methylation (RdDM) is a transcriptional silencing mechanism mediated by small and long noncoding RNAs produced by the plant-specific RNA polymerases Pol IV and Pol V, respectively. Through a chemical genetics screen with a luciferase-based DNA methylation reporter, LUCL, we found that camptothecin, a compound with anti-cancer properties that targets DNA topoisomerase 1α (TOP1α) was able to de-repress LUCL by reducing its DNA methylation and H3K9 dimethylation (H3K9me2) levels. Further studies with Arabidopsis top1α mutants showed that TOP1α promotes RdDM by facilitating the production of Pol V-dependent long non-coding RNAs, AGONAUTE4 recruitment and H3K9me2 deposition at transposable elements (TEs). ten bisufite libraries were sequenced

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Xuemei Chen   Thanh T Dinh  Lei Gao 

PROVIDER: E-GEOD-50691 | ArrayExpress | 2014-07-07

SECONDARY ACCESSION(S): GSE50691SRP029707PRJNA218537

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis.

Dinh Thanh Theresa TT   Gao Lei L   Liu Xigang X   Li Dongming D   Li Shengben S   Zhao Yuanyuan Y   O'Leary Michael M   Le Brandon B   Schmitz Robert J RJ   Manavella Pablo A PA   Li Shaofang S   Weigel Detlef D   Pontes Olga O   Ecker Joseph R JR   Chen Xuemei X  

PLoS genetics 20140703 7


RNA-directed DNA methylation (RdDM) and histone H3 lysine 9 dimethylation (H3K9me2) are related transcriptional silencing mechanisms that target transposable elements (TEs) and repeats to maintain genome stability in plants. RdDM is mediated by small and long noncoding RNAs produced by the plant-specific RNA polymerases Pol IV and Pol V, respectively. Through a chemical genetics screen with a luciferase-based DNA methylation reporter, LUCL, we found that camptothecin, a compound with anti-cancer  ...[more]

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