Transcriptomics

Dataset Information

3

Mechanical loading and gene expression - Microarray study


ABSTRACT: Skeletal integrity in humans and animals is maintained by daily mechanical loading. It has been widely accepted that osteocytes function as mechanosensors. Many biochemical signaling molecules are involved in the response of osteocytes to mechanical stimulation. The aim of this study was to identify genes involved in the translation of mechanical stimuli into bone formation. The four-point bending model was used to induce a single period of mechanical loading (comprising 300 cycles (2 Hz) using a peak magnitude of 60 N) on the right tibia, while the contra lateral left tibia served as control. Six hours after loading, the effects of mechanical loading on gene-expression were determined with microarray analysis. Protein expression of differentially regulated genes was evaluated with immunohistochemistry. Nine genes were found to exhibit a significant differential gene expression in LOAD compared to control. MEPE, Garnl1, V2R2B, and QFG TN1 olfactory receptor were up-regulated, and creatine kinase (muscle form), fibrinogen-B beta-polypeptide, monoamine oxidase A, troponin-C and kinesin light chain-C were down-regulated. Validation with real-time RT-PCR analysis confirmed the up regulation of MEPE and the down-regulation of creatine kinase (muscle form) and troponin-C in the loaded tibia. Immunohistochemistry showed that the increase of MEPE protein expression was already detectable six hours after mechanical loading. In conclusion, these genes probably play a role during translation of mechanical stimuli six hours after mechanical loading. The modulation of MEPE expression may indicate a connection between bone mineralization and bone formation after mechanical stimulation. Two groups: LOAD vs contralateral control and SHAM vs contralateral control (n=5/group)

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Nathalie Bravenboer   Marinus A Blankenstein  Johannes H van Beek  Bauke Ylstra  Birgitte T van Rens  Paul Lips  Christianne M Reijnders  Huib W van Essen 

PROVIDER: E-GEOD-50707 | ArrayExpress | 2013-11-21

SECONDARY ACCESSION(S): GSE50707PRJNA218533

REPOSITORIES: GEO, ArrayExpress

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Publications

Increased expression of matrix extracellular phosphoglycoprotein (MEPE) in cortical bone of the rat tibia after mechanical loading: identification by oligonucleotide microarray.

Reijnders Christianne M A CM   van Essen Huib W HW   van Rens Birgitte T T M BT   van Beek Johannes H G M JH   Ylstra Bauke B   Blankenstein Marinus A MA   Lips Paul P   Bravenboer Nathalie N  

PloS one 20131108 11


Skeletal integrity in humans and animals is maintained by daily mechanical loading. It has been widely accepted that osteocytes function as mechanosensors. Many biochemical signaling molecules are involved in the response of osteocytes to mechanical stimulation. The aim of this study was to identify genes involved in the translation of mechanical stimuli into bone formation. The four-point bending model was used to induce a single period of mechanical loading on the right tibia, while the contra  ...[more]

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