Genomics

Dataset Information

298

Role of de novo DNA methyltransferases in hematopoietic stem cells (WGBS)


ABSTRACT: To investigate the global DNA methylation changes in Dnmt3a-KO and Dnmt3ab-dKO HSCs compared to control HSCs, we performed whole genome bisulfite sequencing Mouse hematopoietic stem cell DNA methylation profiles of control, Dnmt3a-KO and Dnmt3ab-dKO HSCs were generated generated by deep sequencing, in duplicate, using Illumina Hiseq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: deqiang sun   Li Wei 

PROVIDER: E-GEOD-50780 | ArrayExpress | 2014-08-19

SECONDARY ACCESSION(S): SRP029902GSE50780PRJNA218940

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


Epigenetic regulation of hematopoietic stem cells (HSCs) ensures lifelong production of blood and bone marrow. Recently, we reported that loss of de novo DNA methyltransferase Dnmt3a results in HSC expansion and impaired differentiation. Here, we report conditional inactivation of Dnmt3b in HSCs either alone or combined with Dnmt3a deletion. Combined loss of Dnmt3a and Dnmt3b was synergistic, resulting in enhanced HSC self-renewal and a more severe block in differentiation than in Dnmt3a-null ce  ...[more]

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