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Gene Expression Analysis of Host Immune Responses in CD11b positive cells isolated from lungs of the UV inactivated SARS-CoV-immunized mice following Lethal SARS-CoV Infection

ABSTRACT: To better understand the biological pathways by which UV inactivated SARS-CoV-induced pulmonary eosinophilia occurs, we examined global transcriptional changes in macrophages from the lungs of mouse. Female BALB/c mice were used at 21 weeks of age. Mice were subcutaneously immunized with UV inactivated SARS-CoV (UV-V) or UV-V and Toll like receptor (TLR) ligands at 6 and 1 weeks prior to mouse-adapted SARS-CoV (n=6 per group). Mice were intranasally challenged with 1E+6 TCID50 in 30μL. MEM was challenged in six mice as control infection. Mice were sacrificed and collected lungs at 1 days after challenge, then CD11b positive cells were isolated from the lungs of these mice. These cells were used for the analysis of microarray.

ORGANISM(S): Mus musculus  

SUBMITTER: A Uda   M Tashiro  Y Tsunetsugu-Yokota  H Hasegawa  Naoko Iwata-Yoshikawa  T Sata  N Iwata-Yoshikawa  N Nagata  Y Sato  S Morikawa 

PROVIDER: E-GEOD-50855 | ArrayExpress | 2014-05-22



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Effects of Toll-like receptor stimulation on eosinophilic infiltration in lungs of BALB/c mice immunized with UV-inactivated severe acute respiratory syndrome-related coronavirus vaccine.

Iwata-Yoshikawa Naoko N   Uda Akihiko A   Suzuki Tadaki T   Tsunetsugu-Yokota Yasuko Y   Sato Yuko Y   Morikawa Shigeru S   Tashiro Masato M   Sata Tetsutaro T   Hasegawa Hideki H   Nagata Noriyo N  

Journal of virology 20140521 15

UNLABELLED:Severe acute respiratory syndrome-related coronavirus (SARS-CoV) is an emerging pathogen that causes severe respiratory illness. Whole UV-inactivated SARS-CoV (UV-V), bearing multiple epitopes and proteins, is a candidate vaccine against this virus. However, whole inactivated SARS vaccine that includes nucleocapsid protein is reported to induce eosinophilic infiltration in mouse lungs after challenge with live SARS-CoV. In this study, an ability of Toll-like receptor (TLR) agonists to  ...[more]

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