Transcriptomics

Dataset Information

37

Gene Expression Analysis of Host Immune Responses in CD11b positive cells isolated from lungs of the UV inactivated SARS-CoV-immunized mice following Lethal SARS-CoV Infection


ABSTRACT: To better understand the biological pathways by which UV inactivated SARS-CoV-induced pulmonary eosinophilia occurs, we examined global transcriptional changes in macrophages from the lungs of mouse. Female BALB/c mice were used at 21 weeks of age. Mice were subcutaneously immunized with UV inactivated SARS-CoV (UV-V) or UV-V and Toll like receptor (TLR) ligands at 6 and 1 weeks prior to mouse-adapted SARS-CoV (n=6 per group). Mice were intranasally challenged with 1E+6 TCID50 in 30μL. MEM was challenged in six mice as control infection. Mice were sacrificed and collected lungs at 1 days after challenge, then CD11b positive cells were isolated from the lungs of these mice. These cells were used for the analysis of microarray.

ORGANISM(S): Mus musculus  

SUBMITTER: A Uda  M Tashiro   Y Tsunetsugu-Yokota   H Hasegawa   Naoko Iwata-Yoshikawa   T Sata   N Iwata-Yoshikawa   N Nagata   Y Sato   S Morikawa    

PROVIDER: E-GEOD-50855 | ArrayExpress | 2014-06-03

SECONDARY ACCESSION(S): GSE50855PRJNA219175

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Effects of Toll-like receptor stimulation on eosinophilic infiltration in lungs of BALB/c mice immunized with UV-inactivated severe acute respiratory syndrome-related coronavirus vaccine.

Iwata-Yoshikawa Naoko N   Uda Akihiko A   Suzuki Tadaki T   Tsunetsugu-Yokota Yasuko Y   Sato Yuko Y   Morikawa Shigeru S   Tashiro Masato M   Sata Tetsutaro T   Hasegawa Hideki H   Nagata Noriyo N  

Journal of virology 20140521 15


Severe acute respiratory syndrome-related coronavirus (SARS-CoV) is an emerging pathogen that causes severe respiratory illness. Whole UV-inactivated SARS-CoV (UV-V), bearing multiple epitopes and proteins, is a candidate vaccine against this virus. However, whole inactivated SARS vaccine that includes nucleocapsid protein is reported to induce eosinophilic infiltration in mouse lungs after challenge with live SARS-CoV. In this study, an ability of Toll-like receptor (TLR) agonists to reduce the  ...[more]

Similar Datasets

| GSE50855 | GEO
2014-06-02 | E-GEOD-44274 | ArrayExpress
2014-11-12 | E-GEOD-52920 | ArrayExpress
| GSE90624 | GEO
| GSE52920 | GEO
2011-09-01 | GSE23955 | GEO
2014-06-03 | E-GEOD-47962 | ArrayExpress
2011-09-14 | E-GEOD-23955 | ArrayExpress
2014-06-03 | E-GEOD-47960 | ArrayExpress
2014-06-03 | E-GEOD-50000 | ArrayExpress