Transcriptomics

Dataset Information

30

Gene expression timecourse in ΔrpaA strain


ABSTRACT: The goal of the experiment was to determine whether gene expression oscillates in the absence of rpaA. It was reported previously (e.g., Takai et al, PNAS 2006) that activity of a handful of individual expression reporters was arrythmic, and we conducted this microarray timecourse to determine whether expression is arrhythmic geneome-wide. Cultures were grown in a turbidostat as described previously (Vijayan et al, PNAS 2009). Cultures were entrained with two consecutive light/dark cycles and released into continuous light at time T = 0. Cultures were samples every 4 hours for 48 h between T = 24 h and T = 72 h, inclusive. Gene expression at each timepoint was compared to the time-averaged gene expression (determined using a pool of equal mass quantities of RNA from all timepoints) using a two-color Agilent microarray.

ORGANISM(S): Synechococcus elongatus PCC 7942  

SUBMITTER: Anna M Puszynska  Joseph S Markson   Joseph Scott Markson   Erin K O'Shea   Joseph R Piechura    

PROVIDER: E-GEOD-50908 | ArrayExpress| 2014-06-03

SECONDARY ACCESSION(S): GSE50908PRJNA219378

REPOSITORIES: GEO, ArrayExpress

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