Transcriptomics

Dataset Information

3

Cavin-3 Dictates the Balance Between ERK and Akt Signaling


ABSTRACT: Cavin-3 is a tumor suppressor protein of unknown function. Using a combination of in vivo knockout and in vitro gain/loss of function approaches, we show that cavin-3 dictates the balance between ERK and Akt signaling. Loss of cavin-3 increases Akt signaling at the expense of ERK, while gain of cavin-3 increases ERK signaling at the expense Akt. Cavin-3 facilitates signal transduction to ERK by anchoring caveolae, a lipid-raft specialization that contains an ERK activation module, to the membrane skeleton of the plasma membrane. Loss of cavin-3 reduces the number of caveolae, thereby separating this ERK activation module from signaling receptors. Loss of cavin-3 promotes Akt signaling through suppression of EGR1 and PTEN. The in vitro consequences of the loss of cavin-3 include induction of Warburg metabolism (aerobic glycolysis), accelerated cell proliferation and resistance to apoptosis. The in vivo consequences of cavin-3 loss are increased lactate production and cachexia. 9 total samples, consisting of 3 cavin-3 siRNA groups (0 days, 3 days and 8 days) one set was untreated, one set was serum starved, one set was serum starved and then treated with EGF for 1 hr.

ORGANISM(S): Homo sapiens  

SUBMITTER: Peter A Michaely   Peter Michaely 

PROVIDER: E-GEOD-50982 | ArrayExpress | 2013-09-24

SECONDARY ACCESSION(S): GSE50982PRJNA219476

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-50982.additional.1.zip Other
E-GEOD-50982.idf.txt Idf
E-GEOD-50982.processed.1.zip Processed
E-GEOD-50982.sdrf.txt Txt
Items per page:
1 - 4 of 4

Similar Datasets

2013-01-01 | S-EPMC3780650 | BioStudies
2010-01-01 | S-EPMC2829113 | BioStudies
2012-01-01 | S-EPMC3500416 | BioStudies
1000-01-01 | S-EPMC1964858 | BioStudies
2014-01-01 | S-EPMC4406223 | BioStudies
1000-01-01 | S-EPMC3999933 | BioStudies
2015-01-01 | S-EPMC4528130 | BioStudies
2009-01-01 | S-EPMC2658630 | BioStudies
2021-12-22 | MSV000088606 | GNPS
2010-01-01 | S-EPMC4297285 | BioStudies