Genomics

Dataset Information

297

The polycomb protein Ezh2 regulates differentiation and plasticity of CD4 T helper type-1 and type-2 cells [ChIP-seq]


ABSTRACT: Following antigen encounter by CD4 T cells, polarizing cytokines induce the expression of master regulators that control differentiation. Inactivation of the histone methyltransferase Ezh2 was found to specifically enhance T-helper (Th)1 and Th2 cell differentiation and plasticity. Ezh2 directly bound and facilitated correct expression of Tbx21 and Gata3 in differentiating Th1 and Th2 cells, accompanied by substantial tri-methylation at lysine 27 of histone 3 (H3K27-Me3). In addition, Ezh2 deficiency resulted in spontaneous generation of discrete IFN-γ and Th2 cytokine-producing populations in non-polarizing cultures, and under these conditions IFN-γ expression was largely dependent on enhanced expression of the transcription factor Eomesodermin. In vivo, Loss of Ezh2 caused increased pathology in a model of allergic asthma and resulted in progressive accumulation of memory phenotype Th2 cells. This study establishes a functional link between Ezh2 and transcriptional regulation of lineage-specifying genes in terminally differentiated CD4 T cells. Examination of Ezh2 binding in Th1 and Th2 cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Damon Tumes   Kenta Shinoda  Chiaki Iwamura  Koji Tokoyoda  Atsushi Onodera  Shinichiro Motohashi  Toshinori Nakayama  Akane Suzuki  Hiroyuki Hosokawa  Yutaka Suzuki  Yusuke Endo 

PROVIDER: E-GEOD-51079 | ArrayExpress | 2013-09-24

SECONDARY ACCESSION(S): GSE51079SRP030204PRJNA221137

REPOSITORIES: GEO, ArrayExpress, ENA

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