Dataset Information


The polycomb protein Ezh2 regulates differentiation and plasticity of CD4 T helper type-1 and type-2 cells [ChIP-seq]

ABSTRACT: Following antigen encounter by CD4 T cells, polarizing cytokines induce the expression of master regulators that control differentiation. Inactivation of the histone methyltransferase Ezh2 was found to specifically enhance T-helper (Th)1 and Th2 cell differentiation and plasticity. Ezh2 directly bound and facilitated correct expression of Tbx21 and Gata3 in differentiating Th1 and Th2 cells, accompanied by substantial tri-methylation at lysine 27 of histone 3 (H3K27-Me3). In addition, Ezh2 deficiency resulted in spontaneous generation of discrete IFN-γ and Th2 cytokine-producing populations in non-polarizing cultures, and under these conditions IFN-γ expression was largely dependent on enhanced expression of the transcription factor Eomesodermin. In vivo, Loss of Ezh2 caused increased pathology in a model of allergic asthma and resulted in progressive accumulation of memory phenotype Th2 cells. This study establishes a functional link between Ezh2 and transcriptional regulation of lineage-specifying genes in terminally differentiated CD4 T cells. Examination of Ezh2 binding in Th1 and Th2 cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Damon Tumes   Kenta Shinoda  Chiaki Iwamura  Koji Tokoyoda  Atsushi Onodera  Shinichiro Motohashi  Toshinori Nakayama  Akane Suzuki  Hiroyuki Hosokawa  Yutaka Suzuki  Yusuke Endo 

PROVIDER: E-GEOD-51079 | ArrayExpress | 2013-09-24



Dataset's files

Action DRS
E-GEOD-51079.idf.txt Idf Processed Processed Processed
E-GEOD-51079.sdrf.txt Txt
Items per page:
1 - 5 of 5

Similar Datasets

2013-09-11 | E-GEOD-50729 | ArrayExpress
2015-04-23 | E-GEOD-66261 | ArrayExpress
2011-03-31 | E-GEOD-28292 | ArrayExpress
2013-04-19 | E-GEOD-46185 | ArrayExpress
2015-11-12 | E-GEOD-60678 | ArrayExpress
2009-06-06 | E-MEXP-2153 | ArrayExpress
2012-01-05 | E-GEOD-32864 | ArrayExpress
2013-12-31 | E-GEOD-51344 | ArrayExpress
2010-06-18 | E-GEOD-22104 | ArrayExpress
2010-07-07 | E-GEOD-22081 | ArrayExpress