Dataset Information


Expression data from E12.5 and E14.5 mouse embryonic gonad of wild type (WT) and Wnt-4 knock-out (KO) mice. [Mouse430_2]

ABSTRACT: Wnt-4 signaling is critical for embryonic female sexual development. When Wnt-4 gene is deleted during embryonic development, the knock-out females present a partial sex reversal. We used microarrays to detail the global programme of gene expression underlying sexual development and identified distinct classes of up and down-regulated genes during this process. Mouse embryonic gonad from wild type and Wnt-4 knock-out mice (male and female) were selected at two stages of sex determination for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of embryonic gonad at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected embryos according to morphological criteria at two time-points: E 12.5 and E14.5.

ORGANISM(S): Mus musculus  

SUBMITTER: Seppo Vainio   Florence Naillat 

PROVIDER: E-GEOD-51089 | ArrayExpress | 2015-06-30



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Identification of the genes regulated by Wnt-4, a critical signal for commitment of the ovary.

Naillat Florence F   Yan Wenying W   Karjalainen Riikka R   Liakhovitskaia Anna A   Samoylenko Anatoly A   Xu Qi Q   Sun Zhandong Z   Shen Bairong B   Medvinsky Alexander A   Quaggin Susan S   Vainio Seppo J SJ  

Experimental cell research 20150130 2

The indifferent mammalian embryonic gonad generates an ovary or testis, but the factors involved are still poorly known. The Wnt-4 signal represents one critical female determinant, since its absence leads to partial female-to-male sex reversal in mouse, but its signalling is as well implicated in the testis development. We used the Wnt-4 deficient mouse as a model to identify candidate gonadogenesis genes, and found that the Notum, Phlda2, Runx-1 and Msx1 genes are typical of the wild-type ovar  ...[more]

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