Transcriptomics

Dataset Information

355

Transcription profiling by high throughput sequencing of Arabidopsis IBH1 and IBL1 gain-of-function mutants and ibh1 and ibl1 loss-of-function mutants


ABSTRACT: To understand how atypical bHLH, INCREASED LEAF INCLINATION1 (ILI1)-BINDING bHLH-1 (IBH1) (At2g43060), and close homologue, IBH1-like1 (IBL1) (At4g30410), interact to regulate cell elongation, genome-wide RNA-Seq expression analyses of IBH1 and IBL1 gain-(IBH1OE, IBL1OE) and loss-of-function (ibh1 (SALK 049177), ibl1(SALK 119457)) mutants were conducted. For loss-of-function mutant, homozygous ibh1(SALK 049177) and ibl1(SALK 119457) were compared to wild type (Col). For gain-of-function mutant, homozygous 35Spro:IBH1-GFP and 35Spro:IBL1-GFP were compared to wild type (Col). Total RNAs were extracted from seedling of each genotypes. For each genotype two biological replicates were sequenced.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Miroslava K Zhiponova   Erich Grotewold  Kengo Morohashi  Eugenia Russinova 

PROVIDER: E-GEOD-51119 | ArrayExpress | 2014-03-05

SECONDARY ACCESSION(S): SRP030399GSE51119PRJNA221230

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Helix-loop-helix/basic helix-loop-helix transcription factor network represses cell elongation in Arabidopsis through an apparent incoherent feed-forward loop.

Zhiponova Miroslava K MK   Morohashi Kengo K   Vanhoutte Isabelle I   Machemer-Noonan Katja K   Revalska Miglena M   Van Montagu Marc M   Grotewold Erich E   Russinova Eugenia E  

Proceedings of the National Academy of Sciences of the United States of America 20140206 7


Cell elongation is promoted by different environmental and hormonal signals, involving light, temperature, brassinosteroid (BR), and gibberellin, that inhibit the atypical basic helix-loop-helix (bHLH) transcription factor INCREASED LEAF INCLINATION1 BINDING bHLH1 (IBH1). Ectopic accumulation of IBH1 causes a severe dwarf phenotype, but the cell elongation suppression mechanism is still not well understood. Here, we identified a close homolog of IBH1, IBH1-LIKE1 (IBL1), that also antagonized BR  ...[more]

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