Transcriptomics

Dataset Information

299

A microRNA-directed program of cytotoxic CD8+ T cell differentiation


ABSTRACT: Acquisition of effector properties is a key step in the generation of cytotoxic T lymphocytes (CTLs). Here we show that inflammatory signals regulate Dicer expression in CTL, and that deletion or depletion of Dicer in mouse or human activated CD8+ T cells causes upregulation of perforin, granzyme and effector cytokines. Genome-wide analysis of miRNA changes induced by exposure of differentiating CTLs to IL-2 and inflammatory signals identifies miR-139 and miR-150 as components of a miRNA network that controls perforin, eomesodermin (Eomes) and IL-2Ra expression in differentiating CTLs and whose activity is modulated by IL-2, inflammation and antigenic stimulation. Overall our data show that strong IL-2R and inflammatory signals act through Dicer and miRNAs to control the cytolytic program and other aspects of effector CTL differentiation. Comparison of control and Dicer knock-out CTLs differentiated in vitro; Comparison of wild type CTLs differentiated in vitro with or without inflammatory stimuli; Comparison of effector and memory precursor CTLs isolated from mice infected with LCMV-Armstrong

ORGANISM(S): Mus musculus  

SUBMITTER: Tarmo Äijö   Anjana Rao  Sara Trifari 

PROVIDER: E-GEOD-51393 | ArrayExpress | 2013-12-02

SECONDARY ACCESSION(S): GSE51393SRP031473PRJNA222887

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

MicroRNA-directed program of cytotoxic CD8+ T-cell differentiation.

Trifari Sara S   Pipkin Matthew E ME   Bandukwala Hozefa S HS   Äijö Tarmo T   Bassein Jed J   Chen Runqiang R   Martinez Gustavo J GJ   Rao Anjana A  

Proceedings of the National Academy of Sciences of the United States of America 20131025 46


Acquisition of effector properties is a key step in the generation of cytotoxic T lymphocytes (CTLs). Here we show that inflammatory signals regulate Dicer expression in CTLs, and that deletion or depletion of Dicer in mouse or human activated CD8(+) T cells causes up-regulation of perforin, granzymes, and effector cytokines. Genome-wide analysis of microRNA (miR, miRNA) changes induced by exposure of differentiating CTLs to IL-2 and inflammatory signals identifies miR-139 and miR-150 as compone  ...[more]

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