Transcriptomics

Dataset Information

3

Functional genomics of cactus host shifts in Drosophila mojavensis


ABSTRACT: Understanding the genetic basis of adaptation to novel environments remains one of the major challenges confronting evolutionary biologists. While newly developed genomic approaches hold considerable promise for addressing this overall question, the relevant tools have not often been available in the most ecologically interesting organisms. Our study organism, Drosophila mojavensis, is a cactophilic Sonoran Desert endemic utilizing four different cactus hosts across its geographic range. Its well-known ecology makes it an attractive system in which to study the evolution of gene expression during adaptation. As a cactophile, D. mojavensis oviposits in the necrotic tissues of cacti, therefore exposing larvae and even adults to the varied and toxic compounds of rotting cacti. We have developed a cDNA microarray of D. mojavensis to examine gene expression associated with cactus host use. Using a population from the Baja California population we examined gene expression differences of third instar larvae when reared in two chemically distinct cactus hosts, agria (Stenocereus gummosus, native host) vs. organpipe (S. thurberi, alternative host). We have observed differential gene expression associated with cactus host use in genes involved in metabolism and detoxification. The experiment was composed of 5 sets of dye-flips (rep1-5). Larvae were reared in either necrotic agria or organpipe cactus tissues. They were then collected at the third instar stage and its total RNA extracted.

ORGANISM(S): Drosophila mojavensis  

SUBMITTER: Benjamin G Bitler   Therese A Markow  Thomas D Watts  Luciano Matias Matzkin  Carlos A Machado  Luciano M Matzkin 

PROVIDER: E-GEOD-5148 | ArrayExpress | 2010-07-01

SECONDARY ACCESSION(S): GSE5148PRJNA96547

REPOSITORIES: GEO, ArrayExpress

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Publications

Functional genomics of cactus host shifts in Drosophila mojavensis.

Matzkin Luciano M LM   Watts Thomas D TD   Bitler Benjamin G BG   Machado Carlos A CA   Markow Therese A TA  

Molecular ecology 20061201 14


Understanding the genetic basis of adaptation to novel environments remains one of the major challenges confronting evolutionary biologists. While newly developed genomic approaches hold considerable promise for addressing this overall question, the relevant tools have not often been available in the most ecologically interesting organisms. Our study organism, Drosophila mojavensis, is a cactophilic Sonoran Desert endemic utilizing four different cactus hosts across its geographical range. Its w  ...[more]

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