Transcriptomics

Dataset Information

83

Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila


ABSTRACT: Ago1-IP small RNA sequencing from S2R+ cells, with dsRNA knockdown of Dicer1 (and GFP control) One Dicer knockdown sample and one GFP knockdown control.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Jakub Orzechowski Westholm  Jakub Westholm   Eric Lai    

PROVIDER: E-GEOD-51585 | ArrayExpress | 2014-06-03

SECONDARY ACCESSION(S): GSE51585SRP031850PRJNA223639

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila.

Yang Jr-Shiuan JS   Smibert Peter P   Westholm Jakub O JO   Jee David D   Maurin Thomas T   Lai Eric C EC  

Nucleic acids research 20131112 3


Although Dicer is essential for general microRNA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent. Instead, its short pre-miRNA hairpin is 'sliced' by Ago2, then 3'-resected into mature miRNAs. Here, we show that Drosophila cells and animals generate functional small RNAs from mir-451-type precursors. However, their bulk maturation arrests as Ago-cleaved pre-miRNAs, which mostly associate with the RNAi effector AGO2. Routing of pre-mir-451 hairpins to the miRNA effector AGO1 was inhib  ...[more]

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