Dataset Information


Effects of exercise on gene expression level in human monocytes

ABSTRACT: We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in monocytes. Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human #130-091-153 and the autoMACS√ǬÆ Pro Separator). Total RNA was extracted using TRIzol. For this study we used Affymetrix plus 2 (total of 24 chips).


ORGANISM(S): Homo sapiens  

SUBMITTER: Shlomit Radom-Aizik   Dan M Cooper 

PROVIDER: E-GEOD-51835 | ArrayExpress | 2014-02-15



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Impact of brief exercise on circulating monocyte gene and microRNA expression: implications for atherosclerotic vascular disease.

Radom-Aizik Shlomit S   Zaldivar Frank P FP   Haddad Fadia F   Cooper Dan M DM  

Brain, behavior, and immunity 20140111

Physical activity can prevent and/or attenuate atherosclerosis, a disease clearly linked to inflammation. Paradoxically, even brief exercise induces a stress response and increases inflammatory cells like monocytes in the circulation. We hypothesized that exercise would regulate the expression of genes, gene pathways, and microRNAs in monocytes in a way that could limit pro-inflammatory function and drive monocytes to prevent, rather than contribute to, atherosclerosis. Twelve healthy men (22-30  ...[more]

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