Genomics

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The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo


ABSTRACT: High resolution ChIP-seq mapping, supported by in vitro reconstitution studies, suggests that the Cse4 nucleosome is a hemisome that occupies the ~80-bp AT-rich CDEII sequence. H4S47C-anchored cleavage mapping of S. cerevisiae centromeres

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Kristina Krassovsky   Terri D Bryson  Srinivas Ramachandran  Kristin Brogaard  Steven Henikoff  Jonathan Widom  Ji-ping Wang  Jorja Henikoff  Jorja G Henikoff 

PROVIDER: E-GEOD-51949 | ArrayExpress | 2014-04-15

SECONDARY ACCESSION(S): GSE51949SRP032449PRJNA225915

REPOSITORIES: GEO, ArrayExpress, ENA

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The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo.

Henikoff Steven S   Ramachandran Srinivas S   Krassovsky Kristina K   Bryson Terri D TD   Codomo Christine A CA   Brogaard Kristin K   Widom Jonathan J   Wang Ji-Ping JP   Henikoff Jorja G JG  

eLife 20140415


In budding yeast, a single cenH3 (Cse4) nucleosome occupies the ∼120-bp functional centromere, however conflicting structural models for the particle have been proposed. To resolve this controversy, we have applied H4S47C-anchored cleavage mapping, which reveals the precise position of histone H4 in every nucleosome in the genome. We find that cleavage patterns at centromeres are unique within the genome and are incompatible with symmetrical structures, including octameric nucleosomes and (Cse4/  ...[more]

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