Dataset Information


Analysis of DNA methylation changes in normal human hematopoietic stem and progenitor cells

ABSTRACT: DNA methylation analysis on purified human long-term and short-term hematopoietic stem cells (LT-HSC, ST-HSC), common myeloid and megakaryocyte-erythrocyte progenitor cells (CMP, MEP) using HELP arrays. FACS-purified hematopoietic stem and progenitor cell (HSPC) subsets were analyzed for changes in DNA methylation using NimbleGen HELP microarrays. Analysis of DNA methylation of bone marrow-derived HSPC subsets of healthy human donors.

ORGANISM(S): Homo sapiens  

SUBMITTER: Ujunwa C Okoye-Okafor   Britta Will  Maximilian Christopeit  Ross L Levine  Yongkai Mo  Ulrich Steidl  Laura Barreyro  John M Greally  Boris Bartholdy  Amit Verma  Tihomira I Todorova  Ari Melnick  Yiting Yu  Tushar D Bhagat 

PROVIDER: E-GEOD-52151 | ArrayExpress | 2014-02-07



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Acute myeloid leukemia (AML) is characterized by disruption of HSC and progenitor cell differentiation. Frequently, AML is associated with mutations in genes encoding epigenetic modifiers. We hypothesized that analysis of alterations in DNA methylation patterns during healthy HSC commitment and differentiation would yield epigenetic signatures that could be used to identify stage-specific prognostic subgroups of AML. We performed a nano HpaII-tiny-fragment-enrichment-by-ligation-mediated-PCR (na  ...[more]

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