Transcriptomics

Dataset Information

7

Expression data from peritoneal macrophages stimulated with trehalose-6,6'-dimycolate (TDM)


ABSTRACT: Macrophage-inducible C-type lectin (Mincle, Clec4e) is a pathogen sensor that recognizes pathogenic fungi and Mycobactrium tuberculosis. We perfomed microarray analysis using peritoneal macrophages stimulated with TDM, a mycobacterial cell wall glycolipid that is known to be a Mincle ligand. Many chemokine and cytokine genes were upregulated in wildtype macrophages stimulated with TDM. Upregulation of these genes were completely abolishd in Mincle KO macrophages. Peritoneal macrophages from WT and Mincle KO mice were stimulated with TDM or vehicle for 24 h (3 samples each). Microarray analysis was performed using Affymetrix Mouse 430 2.0.

ORGANISM(S): Mus musculus  

SUBMITTER: Takahisa Matsuda   Yoshihiro Ogawa  Chikara Komiya  Takayoshi Suganami  Miyako Tanaka 

PROVIDER: E-GEOD-52338 | ArrayExpress | 2013-11-14

SECONDARY ACCESSION(S): GSE52338PRJNA227446

REPOSITORIES: GEO, ArrayExpress

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Publications


In obesity, a paracrine loop between adipocytes and macrophages augments chronic inflammation of adipose tissue, thereby inducing systemic insulin resistance and ectopic lipid accumulation. Obese adipose tissue contains a unique histological structure termed crown-like structure (CLS), where adipocyte-macrophage crosstalk is known to occur in close proximity. Here we show that Macrophage-inducible C-type lectin (Mincle), a pathogen sensor for Mycobacterium tuberculosis, is localized to macrophag  ...[more]

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