Dataset Information


The DNA Double-Strand Break Response Is Abnormal in Myeloblasts From Patients With Therapy-Related Acute Myeloid Leukemia

ABSTRACT: In order to examine if the upregulation of DNA repair genes on chromosome 8 was associated with the abnormal DSB phenotype observed in trisomy 8 (defined by array CGH or cytogenetics), we compared the mRNA levels of DNA repair genes on chromosome 8 in trisomy 8 t-AML patients versus normal t-AML gammaH2AX responders using gene expression array data. Bone marrow cells taken directly from patients after written informed consent were cryopreserved, RNA extracted, and microarray analysis was performed using Affymetrix U133plus2 chips.


ORGANISM(S): Homo sapiens  

SUBMITTER: Jin Shao   Rigoberto de Jesus Pizarro  Matt Walter  Matthew J Walter  Richard K Wilson  Gongfu Zhao  Meagan A Jacoby  Robert S Fulton  Daniel Koboldt 

PROVIDER: E-GEOD-52478 | ArrayExpress | 2013-11-19



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The DNA double-strand break response is abnormal in myeloblasts from patients with therapy-related acute myeloid leukemia.

Jacoby M A MA   De Jesus Pizarro R E RE   Shao J J   Koboldt D C DC   Fulton R S RS   Zhou G G   Wilson R K RK   Walter M J MJ  

Leukemia 20131205 6

The complex chromosomal aberrations found in therapy-related acute myeloid leukemia (t-AML) suggest that the DNA double-strand break (DSB) response may be altered. In this study we examined the DNA DSB response of primary bone marrow cells from t-AML patients and performed next-generation sequencing of 37 canonical homologous recombination (HR) and non-homologous end-joining (NHEJ) DNA repair genes, and a subset of DNA damage response genes using tumor and paired normal DNA obtained from t-AML p  ...[more]

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