Dataset Information


Gene expression timecourse in wild-type strain

ABSTRACT: The goal of the experiment was to obtain a replicate of the wild-type LL circadian timecourse published in Vijayan et al, PNAS 106: 22564-22568 (2009), in order to identify reproducible circadian genes in LL. Cultures were grown in a turbidostat as described previously (Vijayan et al, PNAS 2009), except that the culture volume was 3 L instead of 4.5 L. Cultures were entrained with two consecutive light/dark cycles and released into continuous light at time T = 0. Cultures were samples every 4 hours from T = 36 h and T = 64 h, inclusive. Gene expression at each timepoint was compared to the time-averaged gene expression (determined using a pool of equal mass quantities of RNA from all timepoints) using a two-color Agilent microarray. Timepoint T = 52 h is omitted due to poor data quality.

ORGANISM(S): Synechococcus elongatus PCC 7942 = FACHB-805  

SUBMITTER: Joseph Scott Markson   Vikram Vijayan 

PROVIDER: E-GEOD-52486 | ArrayExpress | 2014-03-06



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Circadian control of global gene expression by the cyanobacterial master regulator RpaA.

Markson Joseph S JS   Piechura Joseph R JR   Puszynska Anna M AM   O'Shea Erin K EK  

Cell 20131201 6

The cyanobacterial circadian clock generates genome-wide transcriptional oscillations and regulates cell division, but the underlying mechanisms are not well understood. Here, we show that the response regulator RpaA serves as the master regulator of these clock outputs. Deletion of rpaA abrogates gene expression rhythms globally and arrests cells in a dawn-like expression state. Although rpaA deletion causes core oscillator failure by perturbing clock gene expression, rescuing oscillator functi  ...[more]

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