Genomics

Dataset Information

2

The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [HiSeq]


ABSTRACT: We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K5 10R-Chd1 decreases nucleosome turnover at the promoter and increases turnover within the gene body. 10 Illumina sequencing samples and 4 Nimblegen array samples

SUBMITTER: Mark Groudine   Peter J Skene  Steven Henikoff  Jorja Henikoff  Aaron E Hernandez 

PROVIDER: E-GEOD-52501 | ArrayExpress | 2014-04-15

SECONDARY ACCESSION(S): SRP033123GSE52501PRJNA229126

REPOSITORIES: GEO, ArrayExpress, ENA

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E-GEOD-52501.idf.txt Idf
E-GEOD-52501.processed.1.zip Processed
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Publications

The nucleosomal barrier to promoter escape by RNA polymerase II is overcome by the chromatin remodeler Chd1.

Skene Peter J PJ   Hernandez Aaron E AE   Groudine Mark M   Henikoff Steven S  

eLife 20140415


RNA polymerase II (PolII) transcribes RNA within a chromatin context, with nucleosomes acting as barriers to transcription. Despite these barriers, transcription through chromatin in vivo is highly efficient, suggesting the existence of factors that overcome this obstacle. To increase the resolution obtained by standard chromatin immunoprecipitation, we developed a novel strategy using micrococcal nuclease digestion of cross-linked chromatin. We find that the chromatin remodeler Chd1 is recruite  ...[more]

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