Transcriptomics

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Enhanced Direct Conversion into Hepatocyte-like Cells by Accelerated Sequential Epigenetic Events using Additional Stem Cell Factors


ABSTRACT: Recent studies have shown that defined sets of transcription factors could directly convert fibroblasts into induced hepatocytes (iHeps). However, the underlying mechanism of direct conversion process toward a hepatic lineage is largely unknown. Here, we report that the direct conversion kinetics from fibroblasts into iHeps throughout screening multiple additional factors that potentially rescue the delayed kinetics of MET and hepatic program. Mouse embryonic fibroblasts (MEFs) were efficiently converted into iHeps in the presence of c-Myc and Klf4 (CK), the activators for MET process, with the previously defined sets of hepatic transcription factors, resulting in remarkably improved generation of iHeps. Furthermore, in the presence of CK, Hnf4α alone could convert fibroblasts into iHeps within 5 days with a relatively higher efficiency. Cells transduced with different combinations of factors were cultured in standard Hep medium. Epithelial colonies were observed within 5 days with much higher numbers in the presence of additional factor, c-Myc and Klf4, compared to control group, indicating the number of epithelial colony was dramatically increased in the presence of additional stem cell factors

ORGANISM(S): Mus musculus  

SUBMITTER: Kyungtae Lim  Dong W Han   Kyung T Lim    

PROVIDER: E-GEOD-52566 | ArrayExpress | 2013-12-03

SECONDARY ACCESSION(S): GSE52566PRJNA230406

REPOSITORIES: GEO, ArrayExpress

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