Transcriptomics

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Leishmania major modulates autophagy in host macrophages during intracellular differentiation


ABSTRACT: Autophagy generally participates in innate immunity by elimination of intracellular pathogens. However, many of them developed successful strategies to counteract their autolysosomal digestion and lastly to exploit this catabolic cellular process. Protozoan parasites of the genus Leishmania are the causative agent of leishmaniasis, one of the 13 most important tropical diseases. Leishmania persists as endo-parasite in host macrophages, where it uses multiple strategies to manipulate the microbicidal host cell functions and to escape from the host immune system. Understanding how Leishmania interacts with host macrophages during uptake, differentiation, intracellular replication, and release might be the key to develop new drugs in target-directed approaches to treat patient with leishmaniasis. Here, we generated expression profiles from bone marrow-derived macrophages (BMDM) at 1h and 24h post infection (p.i.) with Leishmania major and respective controls.

ORGANISM(S): Mus musculus  

SUBMITTER: Heike Weber   Benjamin Frank  Claus Juergen Scholz  Uta Schurigt  Claus J Scholz 

PROVIDER: E-GEOD-52624 | ArrayExpress | 2015-08-03

SECONDARY ACCESSION(S): GSE52624PRJNA229489

REPOSITORIES: GEO, ArrayExpress

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Publications

Autophagic digestion of Leishmania major by host macrophages is associated with differential expression of BNIP3, CTSE, and the miRNAs miR-101c, miR-129, and miR-210.

Frank Benjamin B   Marcu Ana A   de Oliveira Almeida Petersen Antonio Luis AL   Weber Heike H   Stigloher Christian C   Mottram Jeremy C JC   Scholz Claus Juergen CJ   Schurigt Uta U  

Parasites & vectors 20150731


Autophagy participates in innate immunity by eliminating intracellular pathogens. Consequently, numerous microorganisms have developed strategies to impair the autophagic machinery in phagocytes. In the current study, interactions between Leishmania major (L. m.) and the autophagic machinery of bone marrow-derived macrophages (BMDM) were analyzed.BMDM were generated from BALB/c mice, and the cells were infected with L. m. promastigotes. Transmission electron microscopy (TEM) and electron tomogra  ...[more]

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