Dataset Information


ChIP-Seq analysis of PU.1, BATF, and JunB in murine BMDCs

ABSTRACT: Purpose: The purpose of this study is to identify the genome-wide binding sites for IRF4 interaction partners PU.1, BATF, and JunB in dendritic cells. These ChIP-seq data were integrated with gene expression analysis in IRF4-sufficient and -deficient BMDCs in order to assemble an IRF4 gene regulatory network. Hematopoietic bone marrow progenitors from C57BL/6 mice were differentiated with GM-CSF and IL-4 for 5 days. On day 6, BMDCs were stimulated for 6 hours with 100ng/ml LPS. Fixed chromatin was immunoprecipitated with anti-PU.1, BATF, and JunB antibodies and subjected to high-throughput sequencing. The sequencing data for the input DNA was previously submitted as GSM999807.

ORGANISM(S): Mus musculus  

SUBMITTER: Jason A Hackney   Wyne P Lee  Ira Mellman  Jason DeVoss  Cecile Chalouni  Meijuan Zhou  Aly A Khan  Lelia Delamarre  Justin Lesch  Bryan Vander Lugt  Min Xu  Zora Modrusan  Harinder Singh  Smita Agrawal  Summer Park  Chauncey J Spooner 

PROVIDER: E-GEOD-52772 | ArrayExpress | 2013-12-22



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CD11b(+) dendritic cells (DCs) seem to be specialized for presenting antigens via major histocompatibility (MHC) class II complexes to stimulate helper T cells, but the genetic and regulatory basis for this is not established. Conditional deletion of Irf4 resulted in loss of CD11b(+) DCs, impaired formation of peptide-MHC class II complexes and defective priming of helper T cells but not of cytotoxic T lymphocyte (CTL) responses. Gene expression and chromatin immunoprecipitation followed by deep  ...[more]

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