Genomics

Dataset Information

95

ChIP-Seq analysis of PU.1, BATF, and JunB in murine BMDCs


ABSTRACT: Purpose: The purpose of this study is to identify the genome-wide binding sites for IRF4 interaction partners PU.1, BATF, and JunB in dendritic cells. These ChIP-seq data were integrated with gene expression analysis in IRF4-sufficient and -deficient BMDCs in order to assemble an IRF4 gene regulatory network. Hematopoietic bone marrow progenitors from C57BL/6 mice were differentiated with GM-CSF and IL-4 for 5 days. On day 6, BMDCs were stimulated for 6 hours with 100ng/ml LPS. Fixed chromatin was immunoprecipitated with anti-PU.1, BATF, and JunB antibodies and subjected to high-throughput sequencing. The sequencing data for the input DNA was previously submitted as GSM999807.

ORGANISM(S): Mus musculus  

SUBMITTER: Jason A Hackney  Wyne P Lee   Ira Mellman   Jason DeVoss   Cecile Chalouni   Meijuan Zhou   Aly A Khan   Lelia Delamarre   Justin Lesch   Bryan Vander Lugt   Min Xu   Zora Modrusan   Harinder Singh   Smita Agrawal   Summer Park   Chauncey J Spooner    

PROVIDER: E-GEOD-52772 | ArrayExpress | 2014-04-15

SECONDARY ACCESSION(S): GSE52772SRP033729PRJNA231214

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


CD11b(+) dendritic cells (DCs) seem to be specialized for presenting antigens via major histocompatibility (MHC) class II complexes to stimulate helper T cells, but the genetic and regulatory basis for this is not established. Conditional deletion of Irf4 resulted in loss of CD11b(+) DCs, impaired formation of peptide-MHC class II complexes and defective priming of helper T cells but not of cytotoxic T lymphocyte (CTL) responses. Gene expression and chromatin immunoprecipitation followed by deep  ...[more]

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