Transcriptomics

Dataset Information

3

The effect of miR196a of overall gene expression in oral keratinocytes


ABSTRACT: We wished to assess the effect of miR196a on overall gene expression in reducing it in high expressing epithelial cells (D19 and B16) using antimiR196a and over expressing it in low-expressing cells (OKF4) using pre-miR196a. We transfected anti miR196a in to D19 and B16 oral keratinocytes which express high levels of miR196a in triplicate cultures, harvesting 24h after transfection. We transfected pre-miR196 in okf4 immorualised normal oral keratinocytes in duplicate culture. Successful transfection was ensured by qPCR. 16 Samples in total. 2 high expressing cells: control and anti-miR transfected, in triplicate. 1 low expressing cell: control and premiR transfected, in duplicate

ORGANISM(S): Homo sapiens  

SUBMITTER: Daniel W Lambert   Keith David Hunter  Keith D Hunter 

PROVIDER: E-GEOD-52810 | ArrayExpress | 2013-11-28

SECONDARY ACCESSION(S): GSE52810PRJNA230116

REPOSITORIES: GEO, ArrayExpress

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