Transcriptomics,Multiomics

Dataset Information

16

The PDZ-binding motif of SARS-CoV envelope protein is a determinant of viral pathogenesis


ABSTRACT: A recombinant SARS-CoV lacking the envelope (E) protein is attenuated in vivo. Here we report that E protein PDZ-binding motif (PBM), a domain involved in protein-protein interactions, is a major virulence determinant in vivo. Elimination of SARS-CoV E protein PBM by using reverse genetics led to attenuated viruses (SARS-CoV-mutPBM) and to a reduction in the deleterious exacerbate immune response triggered during infection with the parental virus (SARS-CoV-wt). Cellular protein syntenin bound E protein PBM during SARS-CoV infection. Syntenin activates p38 MAPK leading to overexpression of inflammatory cytokines, and we have shown that active p38 MAPK was reduced in lungs of mice infected with SARS-CoVs lacking E protein PBM (SARS-CoV-mutPBM) as compared with the parental virus (SARS-CoV-wt), leading to a decreased expression of inflammatory cytokines and to viral attenuation. Therefore, E protein PBM is a virulence factor that activates pathogenic immune response most likely by using syntenin as a mediator of p38 MAPK induced inflammation. Three biological replicates were independently hybridized (one channel per slide) for each sample type (SARS-CoV-wt, SARS-CoV-mutPBM, Mock). Slides were Sure Print G3 Agilent 8x60K Mouse (G4852A-028005)

OTHER RELATED OMICS DATASETS IN: PRJNA230465

ORGANISM(S): Mus Musculus

SUBMITTER: Juan Carlos Oliveros   Luis Enjuanes  Marta L DeDiego  Raul Fernández-Delgado  José A Regla-Nava  José L Nieto-Torres  José M Jiménez-Guardeño  Carlos Castaño-Rodríguez 

PROVIDER: E-GEOD-52920 | ArrayExpress | 2014-10-31

SECONDARY ACCESSION(S): GSE52920PRJNA230465

REPOSITORIES: GEO, ArrayExpress

Dataset's files

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E-GEOD-52920.idf.txt Idf
E-GEOD-52920.processed.1.zip Processed
E-GEOD-52920.raw.1.zip Raw
E-GEOD-52920.sdrf.txt Txt
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Publications

The PDZ-binding motif of severe acute respiratory syndrome coronavirus envelope protein is a determinant of viral pathogenesis.

Jimenez-Guardeño Jose M JM   Nieto-Torres Jose L JL   DeDiego Marta L ML   Regla-Nava Jose A JA   Fernandez-Delgado Raul R   Castaño-Rodriguez Carlos C   Enjuanes Luis L  

PLoS pathogens 20140814 8


A recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) lacking the envelope (E) protein is attenuated in vivo. Here we report that E protein PDZ-binding motif (PBM), a domain involved in protein-protein interactions, is a major determinant of virulence. Elimination of SARS-CoV E protein PBM by using reverse genetics caused a reduction in the deleterious exacerbation of the immune response triggered during infection with the parental virus and virus attenuation. Cellular protein s  ...[more]

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