Transcriptomics

Dataset Information

5

Methylation profiling of the chimpanzee CNTNAP2 gene in brain using high-resolution tiling arrays.


ABSTRACT: Accelerated brain development is a unique feature of the human species. Not only the size but also morphology, in particular the connections between frontal cortex and basal ganglia distinguish the human brain from great apes and other primates. Recent findings suggest that structural features which may be important for language acquisition are influenced by FOXP2, key regulator of CNTNAP2. CNTNAP2 is one of the largest genes in the chimpanzee genome, encompassing 2.5 Mb. It encodes a neurexin with essential roles in the vertebrate nervous system. The aim of our study was to compare the methylation patterns of CNTNAP2 in human and chimpanzee brains, assuming that epigenetic regulation is essential for brain development and human language abilities. To this end, we designed a NimbleGen tiling array covering the entire chimpanzee CNTNAP2 gene plus 0.1 Mb up- and downstream flanking sequence with an average resolution of 13 bp. Methylated DNA ImmunoPreciptation (MeDIP) was used to enrich cytosine-methylated DNA fragments for downstream analysis with high-resolution tiling arrays. MeDIP-based CNTNAP2 methylation profiling

ORGANISM(S): Pan troglodytes  

SUBMITTER: Thomas Haaf   Nady el Hajj  Claus Juergen Scholz  Eberhard Schneider  Claus J Scholz 

PROVIDER: E-GEOD-52949 | ArrayExpress | 2014-02-20

SECONDARY ACCESSION(S): GSE52949PRJNA230522

REPOSITORIES: GEO, ArrayExpress

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Publications

Widespread differences in cortex DNA methylation of the "language gene" CNTNAP2 between humans and chimpanzees.

Schneider Eberhard E   El Hajj Nady N   Richter Steven S   Roche-Santiago Justin J   Nanda Indrajit I   Schempp Werner W   Riederer Peter P   Navarro Bianca B   Bontrop Ronald E RE   Kondova Ivanela I   Scholz Claus Jürgen CJ   Haaf Thomas T  

Epigenetics 20140116 4


CNTNAP2, one of the largest genes in the human genome, has been linked to human-specific language abilities and neurodevelopmental disorders. Our hypothesis is that epigenetic rather than genetic changes have accelerated the evolution of the human brain. To compare the cortex DNA methylation patterns of human and chimpanzee CNTNAP2 at ultra-high resolution, we combined methylated DNA immunoprecipitation (MeDIP) with NimbleGen tiling arrays for the orthologous gene and flanking sequences. Approxi  ...[more]

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