Transcriptomics

Dataset Information

297

Small RNA deep-sequencing with oxidation/β-elimination with age in Drosophila


ABSTRACT: Drosophila miRNAs show distinct change in isoform distribution pattern with age. Some miRNAs show accumulation of the short isoforms, while other miRNAs show the accumulation of the long isoforms with age. The increase of the long isoforms of some miRNAs reflects increased 2'-O-methylated miRNA isoforms with age. This raised a question on whether additional miRNAs show increased 2'-O-methylated miRNA isoforms with age. To investigate change in 2'-O-methylated small RNA population with age, we performed small RNA deep-sequencing of oxidized or non-oxidized small RNAs at 3d and 30d in Drosophila. This analysis revealed change in global pattern of 2'-O-methylated miRNA isoforms with age. 3d and 30d wild-type male flies were collected. Total RNA was prepared with Trizol reagent, followed by oxidation/β-elimination assay or the control treatment (no-oxidation). Small RNA libraries were prepared using Illumina's TruSeq small RNA sample preparation kit (#RS-200-0012, Illumina, Inc. San Diego, CA), following the manufacturer's protocol. The libraries were multiplexed and sequenced on HiSeq2000 platform (Illumina).

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Andrey Grigoriev   Nancy M Bonini  Ammar Naqvi  Masashi Abe 

PROVIDER: E-GEOD-52960 | ArrayExpress | 2014-01-01

SECONDARY ACCESSION(S): GSE52960SRP033488PRJNA230556

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Impact of age-associated increase in 2'-O-methylation of miRNAs on aging and neurodegeneration in Drosophila.

Abe Masashi M   Naqvi Ammar A   Hendriks Gert-Jan GJ   Feltzin Virzhiniya V   Zhu Yongqing Y   Grigoriev Andrey A   Bonini Nancy M NM  

Genes & development 20140101 1


MicroRNAs (miRNAs) are 20- to ∼24-nucleotide (nt) small RNAs that impact a variety of biological processes, from development to age-associated events. To study the role of miRNAs in aging, studies have profiled the levels of miRNAs with time. However, evidence suggests that miRNAs show heterogeneity in length and sequence in different biological contexts. Here, by examining the expression pattern of miRNAs by Northern blot analysis, we found that Drosophila miRNAs show distinct isoform pattern c  ...[more]

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