Genomics

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BAF155 methylation at R1064 creates unique chromatin association patterns


ABSTRACT: CARM1, a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drastic phenotypic and biochemical changes. The CARM1 KO cell lines enabled identification of novel CARM1 substrates, notably the SWI/SNF core subunit BAF155. Methylation of BAF155 at R1064 was found to be an independent prognostic biomarker for cancer recurrence and to regulate breast cancer cell migration and metastasis. Further, CARM1-mediated BAF155 methylation affects gene expression by directing methylated BAF155 to unique chromatin regions (e.g., c-Myc pathway genes). Collectively our studies uncover a mechanism by which BAF155 acquires tumorigenic functions via arginine methylation. Examination of methylation of BAF155 (R1064) in breast cancer cells

ORGANISM(S): Homo sapiens  

SUBMITTER: Mark Meyer   Lu Wang  Wei Xu  J W Pike 

PROVIDER: E-GEOD-52964 | ArrayExpress | 2014-03-28

SECONDARY ACCESSION(S): GSE52964SRP033492PRJNA230559

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

CARM1 methylates chromatin remodeling factor BAF155 to enhance tumor progression and metastasis.

Wang Lu L   Zhao Zibo Z   Meyer Mark B MB   Saha Sandeep S   Yu Menggang M   Guo Ailan A   Wisinski Kari B KB   Huang Wei W   Cai Weibo W   Pike J Wesley JW   Yuan Ming M   Ahlquist Paul P   Xu Wei W  

Cancer cell 20140101 1


Coactivator-associated arginine methyltransferase 1 (CARM1), a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drastic phenotypic and biochemical changes. The CARM1 KO cell lines enabled identification of CARM1 substrates, notably the SWI/SNF core subunit BAF155. Methylation of B  ...[more]

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