Transcriptomics

Dataset Information

296

Integrative AUF1 PAR-CLIP analysis uncovers AUF1 roles in translation and genome integrity (RNA-Seq 1)


ABSTRACT: We report that AUF1 modulates global mRNA stability and translation, in turn promoting the maintenance of DNA integrity. Please see individual series. For AUF1 PAR-CLIP, the four isoforms of AUF1 (p37, p40, p42, and p45) tagged with a Flag epitope were expressed in HEK293 cells. For total RNA-Seq HEK293 cells were transfected with Control siRNA, AUF1 siRNA, Empty Vector, Flag-AUF1 p37, p40, p42, or p45 as well as WI-38 cells were collected at PDL 15 and 55 and also transfected with Control siRNA, AUF1 siRNA, HuR siRNA. For Ribo-Seq HeLa cells were transfected with Control siRNA, AUF1 siRNA, or HuR siRNA.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kevin G Becker   Janice Barnes  Vidisha Tripathi  Thomas Tuschl  Michele K Evans  Subramanya Srikantan  Min-Ju Kang  William H Wood  Ioannis Grammatikakis  Supriyo De  Jennifer L Martindale  Nicole N Hooten  Nicholas T Ingolia  Xiaoling Yang  Jiyoung Kim  Markus Hafner  Kotb Abdelmohsen  Kannanganattu V Prasanth  Myriam Gorospe  Je-Hyun Yoon 

PROVIDER: E-GEOD-52973 | ArrayExpress | 2014-11-04

SECONDARY ACCESSION(S): GSE52973SRP033498PRJNA230611

REPOSITORIES: GEO, ArrayExpress, ENA

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