Integrative AUF1 PAR-CLIP analysis uncovers AUF1 roles in translation and genome integrity (Ribosome)
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ABSTRACT: We report that AUF1 modulates global mRNA stability and translation, in turn promoting the maintenance of DNA integrity. Please see individual series. In short, For AUF1 PAR-CLIP, the four isoforms of AUF1 (p37, p40, p42, and p45) tagged with a Flag epitope were expressed in HEK293 cells. For total RNA-Seq HEK293 cells were transfected with Control siRNA, AUF1 siRNA, Empty Vector, Flag-AUF1 p37, p40, p42, or p45 as well as WI-38 cells were collected at PDL 15 and 55 and also transfected with Control siRNA, AUF1 siRNA, HuR siRNA. For Ribo-Seq HeLa cells were transfected with Control siRNA, AUF1 siRNA, or HuR siRNA.
ORGANISM(S): Homo sapiens
SUBMITTER:
Vidisha Tripathi Kevin G Becker Janice Barnes Thomas Tuschl Michele K Evans Subramanya Srikantan Min-Ju Kang William H Wood Ioannis Grammatikakis Supriyo De Jennifer L Martindale Nicole N Hooten Nicholas T Ingolia Xiaoling Yang Jiyoung Kim Kotb Abdelmohsen Markus Hafner Kannanganattu V Prasanth Myriam Gorospe Je-Hyun Yoon
PROVIDER: E-GEOD-52976 | ArrayExpress | 2014-11-04
SECONDARY ACCESSION(S): SRP033503GSE52976PRJNA230610
REPOSITORIES: GEO, ArrayExpress, ENA
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