Transcriptomics

Dataset Information

95

Essential role of lncRNA binding for WDR5 maintenance of active chromatin and embryonic stem cell pluripotency


ABSTRACT: Here we delineate the roles of RNA binding for WDR5 function on chromatin state and murine embryonic stem cell (ESC) maintenance. Examination of global transcriptional changes and mapping genome-wide transcription factors occupancy at distinct time points during the transdifferentiation process

ORGANISM(S): Mus musculus  

SUBMITTER: Ryan A Flynn  Yul Yang   Howard Y Chang   Kun Qu    

PROVIDER: E-GEOD-53035 | ArrayExpress | 2014-05-06

SECONDARY ACCESSION(S): GSE53035SRP033544PRJNA230709

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Essential role of lncRNA binding for WDR5 maintenance of active chromatin and embryonic stem cell pluripotency.

Yang Yul W YW   Flynn Ryan A RA   Chen Yong Y   Qu Kun K   Wan Bingbing B   Wang Kevin C KC   Lei Ming M   Chang Howard Y HY  

eLife 20140212


The WDR5 subunit of the MLL complex enforces active chromatin and can bind RNA; the relationship between these two activities is unclear. Here we identify a RNA binding pocket on WDR5, and discover a WDR5 mutant (F266A) that selectively abrogates RNA binding without affecting MLL complex assembly or catalytic activity. Complementation in ESCs shows that WDR5 F266A mutant is unable to accumulate on chromatin, and is defective in gene activation, maintenance of histone H3 lysine 4 trimethylation,  ...[more]

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