Transcriptomics

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Profiling of miRNA expression of HeLa cells infected with Salmonella Typhimurium


ABSTRACT: We identified miRNAs differentially regulated upon Salmonella infection by comparative deep-sequencing analysis of cDNA libraries prepared from the small RNA population (10–29 nt) of HeLa cells infected with Salmonella (20 hpi) and mock-treated cells. Considering that at a MOI of 25 Salmonella is internalized in only 10-15% of the HeLa cells, we separated the fraction of cells which had internalized Salmonella (Salmonella+) from the bystander fraction (Salmonella-) by fluorescence-activated cell sorting (FACS), and extended the analysis of miRNA changes to these samples. Interestingly, we observed that Salmonella infection induces a significant decrease in the expression of all the detected members of the miR-15 family miRNA profiles of HeLa cells infected with Salmonella Typhimurium were generated by deep sequencing, using Illumina HiSeq2000.

ORGANISM(S): Homo sapiens  

SUBMITTER: Mauro Giacca  Claire Maudet   Malvika Sharan   Miguel Mano   Ana Eulalio   Konrad U Förstner   Ushasree Sunkavalli   Konrad U. Förstner    

PROVIDER: E-GEOD-53278 | ArrayExpress | 2014-07-18

SECONDARY ACCESSION(S): GSE53278SRP034007PRJNA231558

REPOSITORIES: GEO, ArrayExpress, ENA

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