Transcriptomics

Dataset Information

240

Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation.


ABSTRACT: Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, we used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. We found that SRSF10 promoted both exon inclusion and exclusion. Functionally, many of SRSF10-verified alternative exons are linked to pathways of stress and apoptosis. Importantly, reconstituted SRSF10 in knockout cells recovered wild-type splicing patterns and considerably rescued the stress-related defects. Together, our results provide mechanistic insight into SRSF10-regulated alternative splicing events in vivo and demonstrate that SRSF10 plays a crucial role in cell survival under stress conditions. RNA-seq for wide type (WT) and SRSF10-deficient (KO) chicken DT40 cells

ORGANISM(S): Gallus gallus  

SUBMITTER: Wenwu Wu   Ying Feng 

PROVIDER: E-GEOD-53354 | ArrayExpress | 2014-01-22

SECONDARY ACCESSION(S): SRP034520GSE53354PRJNA231776

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation.

Zhou Xuexia X   Wu Wenwu W   Li Huang H   Cheng Yuanming Y   Wei Ning N   Zong Jie J   Feng Xiaoyan X   Xie Zhiqin Z   Chen Dai D   Manley James L JL   Wang Hui H   Feng Ying Y  

Nucleic acids research 20140117 6


Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, we used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. We found that SRSF10 promoted both exon inclusion and exclusion. Motif analysis revealed that SRSF10 binding to cassette exons was associated with exon inclusion, whereas the binding of SRSF10 within downstream constitutive exons was associated with exon exclusion. This po  ...[more]

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