Transcriptomics

Dataset Information

2

Suppressio​n of MicroRNA-9 by Mutant EGFR Signaling Induces FOXP1 to Enhance Glioblasto​ma Tumorigeni​city


ABSTRACT: The EGF-receptor (EGFR) is amplified and mutated in glioblastoma (GBM) where its common mutation, (∆EGFR, also called EGFRvIII) has a variety of activities that promote growth and inhibit death, thereby conferring a strong tumor-enhancing effect. This range of activities suggested to us that ∆EGFR might exert its influence through pleiotropic effectors and we hypothesized that microRNAs (miRs) might serve such a function. To test this, we determined the miR profiles of GBM cells with activated wild type EGFR (wtEGFR) and mutant EGFR (∆EGFR) to cells with non-activated EGFR or kinase dead ∆EGFR. To identify miRs regulated by EGFR, RNA from 2 different glioma cell lines (U87 and U373) were hybridized to miR expression arrays and analyzed. Each cell type was engineered to express wild type EGFR (wtEGFR), dead kinase ∆EGFR (DK) or ∆EGFR at elevated levels similar to those observed in primary glioblastomas displaying EGFR overexpression. Parental cells expressing endogenous EGFR and wtEGFR cells stimulated with EGF for 1hr were also included in the analyses.

ORGANISM(S): Homo sapiens  

SUBMITTER: Frank B Furnari  

PROVIDER: E-GEOD-53504 | ArrayExpress | 2013-12-20

SECONDARY ACCESSION(S): GSE53504PRJNA232148

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2014-04-01 | E-GEOD-56350 | ArrayExpress
2012-01-11 | E-GEOD-29707 | ArrayExpress
2013-01-01 | E-GEOD-36901 | ArrayExpress
| GSE96682 | GEO
2015-09-14 | E-GEOD-72468 | ArrayExpress
2011-11-05 | E-GEOD-33442 | ArrayExpress
| GSE57547 | GEO
2009-08-09 | E-GEOD-11729 | ArrayExpress
2012-06-25 | PRD000593 | Pride
2009-07-30 | GSE11729 | GEO