Transcriptomics

Dataset Information

3

Transcription profiling of mouse small intestine intraepithelial lymphocytes (IEL) vs. TCR ab+ CD8b+ and TCR gd+ cells


ABSTRACT: Mouse small intestine intraepithelial lymphocytes (IEL) that express a ab TCR and CD8aa homodimers are an enigmatic T cell subset, as their specificity and in vivo function remain to be defined. To gain insight into the nature of these cells, we performed global gene expression profiling using microarray analysis, combined with PCR and flow cytometry to determine the level of expression of selected genes. Using these methods, TCR ab+ CD8aa IEL were compared to their TCR ab+ CD8b+ and TCR gd+ counterparts. Experiment Overall Design: In this study, the three IEL populations were isolated by cell sorting from a pool of IELs from 20 mice. RNA from each IEL subset was used to probe one microarray. A replicate experiment was performed on a seperate occasion using a new pool of 20 mice and three additional microarrays were used.

ORGANISM(S): Mus musculus  

SUBMITTER: Steve W. Granger 

PROVIDER: E-GEOD-5355 | ArrayExpress | 2008-06-13

SECONDARY ACCESSION(S): GSE5355PRJNA96247

REPOSITORIES: GEO, ArrayExpress

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Publications

Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses.

Denning Timothy L TL   Granger Steve W SW   Mucida Daniel D   Graddy Ryan R   Leclercq Georges G   Zhang Weiguo W   Honey Karen K   Rasmussen Jeffrey P JP   Cheroutre Hilde H   Rudensky Alexander Y AY   Kronenberg Mitchell M  

Journal of immunology (Baltimore, Md. : 1950) 20070401 7


Mouse small intestine intraepithelial lymphocytes (IEL) that express alphabetaTCR and CD8alphaalpha homodimers are an enigmatic T cell subset, as their specificity and in vivo function remain to be defined. To gain insight into the nature of these cells, we performed global gene expression profiling using microarray analysis combined with real-time quantitative PCR and flow cytometry. Using these methods, TCRalphabeta(+)CD8alphaalpha IEL were compared with their TCRalphabeta(+)CD8beta(+) and TCR  ...[more]

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