Genomics

Dataset Information

298

Multiple Overlapping Mammalian Chromatin Remodeling Systems Collaborate Genome-wide at Dynamic Chromatin Transitions (Dnase-Seq)


ABSTRACT: ATP-dependent chromatin remodeling is an essential process required for the dynamic organization of chromatin structure. Here we describe the genome-wide location and activity of three remodeler proteins with diverse physiological functions in the mouse genome: Brg1, Chd4, and Snf2h. The localization patterns of all three proteins significantly overlap with one another and with regions of accessible chromatin. Furthermore, using inducible mutant variants, we demonstrate that the catalytic activity of these proteins contributes to the remodeling of chromatin genome-wide, and that each of these remodelers can independently regulate chromatin reorganization at distinct sites. Most regions require the activity of more than one remodeler to regulate accessibility. These findings provide a dynamic view of chromatin organization, and highlight the differential contributions of remodelers to chromatin maintenance in higher eukaryotes. We examine the genome-wide location and activity of three remodeler proteins (Brg1, CHD4 and Snf2h)

ORGANISM(S): Mus musculus  

SUBMITTER: Myong-Hee Sung   Stephanie A Morris  Gordon Hager  Songjoon Baek 

PROVIDER: E-GEOD-53584 | ArrayExpress | 2013-12-24

SECONDARY ACCESSION(S): GSE53584SRP034690PRJNA232456

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Overlapping chromatin-remodeling systems collaborate genome wide at dynamic chromatin transitions.

Morris Stephanie A SA   Baek Songjoon S   Sung Myong-Hee MH   John Sam S   Wiench Malgorzata M   Johnson Thomas A TA   Schiltz R Louis RL   Hager Gordon L GL  

Nature structural & molecular biology 20131208 1


ATP-dependent chromatin remodeling is an essential process required for the dynamic organization of chromatin structure. Here we describe the genome-wide location and activity of three remodeler proteins with diverse physiological functions in the mouse genome: Brg1, Chd4 and Snf2h. The localization patterns of all three proteins substantially overlap with one another and with regions of accessible chromatin. Furthermore, using inducible mutant variants, we demonstrate that the catalytic activit  ...[more]

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