Transcriptomics

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Decoding the black box of cellulase formation in Hypocrea jecorina by RNA-Seq analysis


ABSTRACT: Hypocrea jecorina (anamorph Trichoderma reesei) is one of the most well studied fungi used in biotechnology industry. This fungus is today a paradigm for the comercial scale production of different plant cell wall degrading enzymes, mainly cellulases and hemicellulases. The objective of this study was to analyze the transcriptional profiling of T. reesei grown in presence of cellulose, sophorose and glucose as the carbon source using RNA-seq approach. T. reesei (QM9414) was grown in Mandels-Andreotti medium, supplemented with 1% of cellulose, 2% of glucose or 1mM of sophorose. The cultures were incubated on an orbital shaker (200 rpm) at 28°C for 24, 48 and 72 hours using cellulose as carbon source; for 24 and 48 hours with glucose as the carbon; and 2, 4 and 6 hours with sophorose as the carbon source. In the latter, the mycelium was previously grown on glycerol for 24 hours and then transferred to 20 mL of Mandels-Andreotti medium without peptone. All experiments were performed in three biological replicates. The resultant mycelia were collected by filtration, frozen and stored at -80°C until RNA extraction. After growth, total RNA was isolated from the mycelia using TRIzol® reagent. RNA-seq experiments were performed by LGC Genomics GmbH (Berlin/Germany) using the platform Illumina/HiSeq2000.

ORGANISM(S): Trichoderma reesei  

SUBMITTER: Roberto do Nascimento Silva  Nilce M Martinez Rossi   Vitor M Faça   Lílian dos Santos Castro   Gabriela F Persinoti   Rafael S Rocha   Andrei S Steindorff   Antonio R Filho   Wellington R Pedersoli   Júlio C Bortolossi   Amanda C Campos Antonieto    

PROVIDER: E-GEOD-53629 | ArrayExpress | 2014-04-01

SECONDARY ACCESSION(S): SRP034709GSE53629PRJNA232512

REPOSITORIES: GEO, ArrayExpress, ENA

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The filamentous fungus Trichoderma reesei is a major producer of lignocellulolytic enzymes utilized by bioethanol industries. However, to achieve low cost second generation bioethanol production on an industrial scale an efficient mix of hydrolytic enzymes is required for the deconstruction of plant biomass. In this study, we investigated the molecular basis for lignocellulose-degrading enzyme production T. reesei during growth in cellulose, sophorose, and glucose.We examined and compared the tr  ...[more]

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