Dataset Information


Expression data of lungs from chickens infected with different H5N1 Avian Influenza Viruses [12 hours post infection]

ABSTRACT: Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3). Chickens were inoculated with CK10 or CK-PAX3 or mock-infected. At 12 hours post infection, lungs were isolated from three chickens per group for RNA extraction and hybridization on Affymetrix microarrays. Samples were named as follows: CK10 (CK10-1,CK10-2,CK10-3), CK-PAX3 (CK-PAX3-1,CK-PAX3-2, CK-PAX3-3), Control (C-1, C-2, C-3).

ORGANISM(S): Gallus gallus  

SUBMITTER: Jiao Hu   Zenglei Hu 

PROVIDER: E-GEOD-53930 | ArrayExpress | 2015-03-04



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PA-X decreases the pathogenicity of highly pathogenic H5N1 influenza A virus in avian species by inhibiting virus replication and host response.

Hu Jiao J   Mo Yiqun Y   Wang Xiaoquan X   Gu Min M   Hu Zenglei Z   Zhong Lei L   Wu Qiwen Q   Hao Xiaoli X   Hu Shunlin S   Liu Wenbo W   Liu Huimou H   Liu Xiaowen X   Liu Xiufan X  

Journal of virology 20150128 8

PA-X is a newly discovered protein that decreases the virulence of the 1918 H1N1 virus in a mouse model. However, the role of PA-X in the pathogenesis of highly pathogenic avian influenza viruses (HPAIV) of the H5N1 subtype in avian species is totally unknown. By generating two PA-X-deficient viruses and evaluating their virulence in different animal models, we show here that PA-X diminishes the virulence of the HPAIV H5N1 strain A/Chicken/Jiangsu/k0402/2010 (CK10) in mice, chickens, and ducks.  ...[more]

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