Transcriptomics

Dataset Information

113

Global Transcriptome Analyses of Mammalian Terminal Erythroid Differentiation


ABSTRACT: Purpose:The purpose of this study is to create unbiased, stage-specific transcriptomes by RNA-seq analyses of pure populations of both murine and human erythroblasts at distinct developmental stages. Methods: Recently developed FACS-based methods (Chen et al, PNAS, Liu et al, Blood, Hu et al Blood) were employed to purify morphologically and functionally discrete populations of cells, each representing specific stages of terminal erythroid differentiation. RNA was prepared from these cells and subjected to RNA-seq analyses. Results: There were vast temporal changes in gene expression across the differentiation stages, with each stage exhibiting unique transcriptomes.Clustering and network analyses revealed that differing stage-specific patterns of expression across differentiation were transcriptionally enriched for genes of differing function. Numerous differences were present between human and murine transcriptomes, with significant variation in the global patterns of gene expression. Conclusions: These data provide a significant resource for studies of normal and perturbed erythropoiesis, allowing a deeper understanding of mechanisms of erythroid development, differentiation, and inherited and acquired disease. Both murine and human erythroblasts at distinct developmental stage mRNA profiles were generated by deep sequencing, in triplicate, using IlluminaHiSeq 2000.

ORGANISM(S): Musculus  

SUBMITTER: Xiuli An  Jingping Hu   Patrick G Gallagher   Jing Liu   Fumin Xue   Vince Schulz   Vincent P Schulz   Jie Li   Kunlu Wu   Narla Mohandas    

PROVIDER: E-GEOD-53983 | ArrayExpress| 2014-04-15

SECONDARY ACCESSION(S): SRP035312GSE53983PRJNA234281

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Global transcriptome analyses of human and murine terminal erythroid differentiation.

An Xiuli X   Schulz Vincent P VP   Li Jie J   Wu Kunlu K   Liu Jing J   Xue Fumin F   Hu Jingping J   Mohandas Narla N   Gallagher Patrick G PG  

Blood 20140317 22


We recently developed fluorescence-activated cell sorting (FACS)-based methods to purify morphologically and functionally discrete populations of cells, each representing specific stages of terminal erythroid differentiation. We used these techniques to obtain pure populations of both human and murine erythroblasts at distinct developmental stages. RNA was prepared from these cells and subjected to RNA sequencing analyses, creating unbiased, stage-specific transcriptomes. Tight clustering of tra  ...[more]

Similar Datasets

2014-04-15 | E-GEOD-53635 | ArrayExpress
2013-08-22 | E-GEOD-25602 | ArrayExpress
2014-05-03 | E-GEOD-48020 | ArrayExpress
2012-06-26 | E-GEOD-22903 | ArrayExpress
2014-12-10 | E-GEOD-54826 | ArrayExpress
2012-03-27 | E-GEOD-14588 | ArrayExpress
2015-05-02 | E-GEOD-62647 | ArrayExpress
2014-06-02 | E-GEOD-32110 | ArrayExpress
2014-06-02 | E-GEOD-22552 | ArrayExpress
2016-01-26 | MSV000079482 | Massive