Dataset Information


H2A.Z marks antisense promoters and has positive effects on antisense transcript levels in budding yeast

ABSTRACT: The histone variant H2A.Z, which has been reported to have both activating and repressive effects on gene expression, is known to occupy nucleosomes at the 5’ ends of protein-coding genes. We now find that H2A.Z is also significantly enriched in gene coding regions and at the 3’ ends of genes in budding yeast, where it co-localises with histone marks associated with active promoters. By comparing H2A.Z binding to global gene expression in budding yeast strains engineered so that normally unstable transcripts are abundant, we show that H2A.Z is required for normal levels of antisense transcripts as well as sense ones. High levels of H2A.Z at antisense promoters are associated with decreased antisense transcript levels when H2A.Z is deleted, indicating that H2A.Z has an activating effect on antisense transcripts. Decreases in antisense transcripts affected by H2A.Z are accompanied by increased levels of paired sense transcripts. Therefore, the effect of H2A.Z on protein coding gene expression is a reflection of its importance for normal levels of both sense and antisense transcripts. Htz1 ChIP-seq in wild-type (WT) and rrp6Δ yeast, along with negative control ChIP-seq in htz1Δ and input control. Strand-specific transcriptomic profiles of WT, htz1Δ, rrp6Δ and htz1Δrrp6Δ. Replicates are present for all samples except the negative and input control ChIP samples.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Catherine B Millar  Thomas J Wood   Muxin Gu   Yanin Naiyachit    

PROVIDER: E-GEOD-54105 | ArrayExpress | 2015-01-10



altmetric image


Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2011-03-01 | E-GEOD-14398 | ArrayExpress
2011-03-01 | GSE14398 | GEO
2012-03-05 | E-GEOD-16977 | ArrayExpress
2009-12-31 | GSE16977 | GEO
| phs000937 | dbGaP
2014-08-13 | E-GEOD-58319 | ArrayExpress
2018-07-23 | GSE101368 | GEO
2017-05-18 | MODEL1511170001 | BioModels Database
2017-05-18 | MODEL1511170002 | BioModels Database
2017-05-18 | MODEL1511170000 | BioModels Database