Transcriptomics

Dataset Information

238

RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe [Illumina Genome Analyzer]


ABSTRACT: RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe. Small RNA library from wild type S. pombe.

ORGANISM(S): Schizosaccharomyces pombe 972h-  

SUBMITTER: Peter Swoboda   Per Kylsten  Agata Smialowska  Jingwen Wang  Karl Ekwall  Ingela Djupedal 

PROVIDER: E-GEOD-54194 | ArrayExpress | 2014-01-23

SECONDARY ACCESSION(S): SRP035550GSE54194PRJNA235985

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe.

Smialowska Agata A   Djupedal Ingela I   Wang Jingwen J   Kylsten Per P   Swoboda Peter P   Ekwall Karl K  

Biochemical and biophysical research communications 20140123 2


RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expres  ...[more]

Similar Datasets

2014-01-23 | E-GEOD-54190 | ArrayExpress
| GSE54194 | GEO
2010-09-25 | GSE24360 | GEO
2012-11-26 | E-GEOD-41634 | ArrayExpress
2013-12-26 | E-GEOD-52444 | ArrayExpress
2014-04-21 | E-GEOD-38636 | ArrayExpress
2012-03-19 | E-GEOD-36113 | ArrayExpress
| GSE66939 | GEO
| GSE66940 | GEO
2010-05-01 | GSE21177 | GEO