Transcriptomics

Dataset Information

92

The Atlas of Chromatoid Body Components [RNA-seq]


ABSTRACT: The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein coding mRNAs and a considerable amount of different non-coding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the requisite transcripts. Haploid spermatids are characterized by a unique, unusually large cytoplasmic granule, the chromatoid body (CB), that emerges during the switch between the meiotic and post-meiotic phases of spermatogenesis. To better understand the role of the CB in male germ cell differentiation, we isolated CBs from mouse testes and revealed its full RNA and protein composition. We showed that the CB is mainly composed of RNA-binding proteins and other proteins involved RNA regulation. The CB was loaded with RNA, including pachytene piRNAs, a diverse set of mRNAs and a number of uncharacterized long non-coding transcripts. The CB was demonstrated to accumulate nascent RNA during all the steps of round spermatid differentiation. Our results revealed the CB as a large germ cell-specific RNP platform that is involved in the control of the highly complex transcriptome of haploid male germ cells. Transcriptome profling of purified chromatoid body, each steps of chromatoid body purification, and sortred round spermatid cells

ORGANISM(S): Mus musculus  

SUBMITTER: ying jin  Jorma Toppari   Noora Kotaja   Karin Sõsta   Aurélie Lardenois   Vasily V Vagin   Molly Hammell   Kaja A Wasik   Matteo Da Ros   Gregory J Hannon   Oliver Meikar   Ying Jin    

PROVIDER: E-GEOD-54286 | ArrayExpress| 2014-04-15

SECONDARY ACCESSION(S): GSE54286SRP035612PRJNA236167

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-04-15 | E-GEOD-54287 | ArrayExpress
2011-09-06 | E-GEOD-30144 | ArrayExpress
| GSE39480 | GEO
2014-06-02 | E-GEOD-39480 | ArrayExpress
| GSE90869 | GEO
2006-05-15 | GSE4711 | GEO
2014-06-03 | E-GEOD-54288 | ArrayExpress
| PRJNA236165 | ENA
2012-03-27 | E-GEOD-4711 | ArrayExpress
| GSE99170 | GEO