Transcriptomics

Dataset Information

5

Identification of genes cooperatively regulated by cytokinin and HD-ZIP III transcription factors


ABSTRACT: In Arabidopsis thaliana, cytokinin responsive B-type ARR transcription factors and HD-ZIP III transcription factors such as REVOLUTA (REV), act cooperatively as master regulators of shoot regeneration. To identify the downstream targets of ARR-HD-ZIP III transcriptional complex, we used an inducible line of REV, 35S::FLAG-GR-rREV, in which FLAG-tagged miR165/6-non-targetable form of REV (rREV)-GR fusion protein was expressed from 35S promoter. DEX treatment induced activation of REV by translocation of FLAG-GR-rREV fusion protein from cytoplasm to the nucleus. We treated 35S::FLAG-GR-rREV seedlings with 6-benzylaminopurine (6-BA, a cytokinin), dexamethasone (DEX), or 6-BA+DEX for 2 hours. Total RNAs were extracted and subjected to Agilent Arabidopsis Gene Expression Microarray analyses. The differentially expressed genes (>1.5-fold, p<0.05) were identified. 10-day-old 35S::FLAG-GR-rREV plants were treated with 6-benzylaminopurine (6-BA), dexamethasone (DEX), or 6-BA+DEX for 2 hours. DEX treatment induced activation of REV by translocation of FLAG-GR-rREV fusion protein from cytoplasm to the nucleus. Total RNA was extracted with RNeasy Mini Kit and hybridized to Agilent Arabidopsis Gene Expression Microarray. Differentially expressed genes were defined by a 1.5-fold expression difference with a P value<0.05. Biological replicates were performed.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Jia-Wei Wang   Tian-Qi Zhang 

PROVIDER: E-GEOD-54469 | ArrayExpress | 2014-01-29

SECONDARY ACCESSION(S): GSE54469PRJNA236588

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

| GSE69411 | GEO
2011-05-01 | GSE25304 | GEO
| GSE42862 | GEO
2011-04-30 | E-GEOD-25304 | ArrayExpress
2011-01-01 | E-GEOD-20175 | ArrayExpress
2007-11-28 | GSE9702 | GEO
2011-03-31 | E-GEOD-24169 | ArrayExpress
2010-06-25 | E-GEOD-3399 | ArrayExpress
2008-06-16 | E-GEOD-9702 | ArrayExpress
2016-07-01 | E-GEOD-70796 | ArrayExpress