Transcriptomics

Dataset Information

3

MiR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy (microRNA)


ABSTRACT: MicroRNA (miRNA) expression profiling identified miR-638 as one of the most significantly overexpressed miRNAs in metastatic lesions compared with primary melanomas. miR-638 enhanced the tumourigenic properties of melanoma cells in vitro and lung colonization in vivo. mRNA expression profiling of miR-638 and antagomir-transduced cells identified new candidate genes as miR-638 targets, the majority of which is involved in p53-mediated apoptosis regulation. miR-638 depletion stimulated expression of p53 and its downstream target genes and induced apoptosis and autophagy in melanoma cells. miR-638 promoter analysis revealed transcription factor associated protein 2-α (TFAP2A) as a direct negative regulator of miR-638. Further analyses provided strong evidence for a double negative regulatory feedback loop between miR-638 and TFAP2A. Taken together, miR-638 may support melanoma progression by suppressing p53-mediated apoptosis pathways and by targeting the transcriptional repressor TFAP2A. TaqMan® low-density arrays (TLDA; human microRNA Cards A v2.1 & B v2.0, Applied Biosystems, Darmstadt, Germany) were used for measuring the expression of 667 human miRNAs in primary melanomas (PM, n=8), lymph node metastases (LNM, n=9) or distant cutaneous metastases (DCM, n=10).

ORGANISM(S): Homo sapiens  

SUBMITTER: Marianne Schauer   Manfred Kunz  Sandra Franz  Yvonne Raatz  Anja Saalbach  Dirk Schadendorf  Jan C Simon  Animesh Bhattacharya  Olaf Wolkenhauer  Thomas Magin  Madeleine Schönherr  Julio Vera  Ulf Anderegg  Tina Kottek  Ulf Schmitz 

PROVIDER: E-GEOD-54492 | ArrayExpress | 2014-01-30

SECONDARY ACCESSION(S): GSE54492PRJNA236647

REPOSITORIES: GEO, ArrayExpress

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