Transcriptomics

Dataset Information

182

Transcription profiling by array of Arabidopsis overexpressing rice topoisomerase 6 genes


ABSTRACT: 10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing OsTOP6A3 and OsTOP6B) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Experiment Overall Design: The experiment involves the study of differential gene expression in transgenic Arabidopsis seedlings overexpressing rice topoisomerase 6 subunit A (OsTOP6A3) and subunit B (OsTOP6B) cDNAs as compared to wild-type.Two biological replicates for each sample were used for microarray analysis.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Mukesh Jain  

PROVIDER: E-GEOD-5465 | ArrayExpress | 2008-06-13

SECONDARY ACCESSION(S): GSE5465PRJNA95977

REPOSITORIES: GEO, ArrayExpress

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Publications

Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants.

Jain Mukesh M   Tyagi Akhilesh K AK   Khurana Jitendra P JP  

The FEBS journal 20061201 23


DNA topoisomerase 6 (TOP6) belongs to a novel family of type II DNA topoisomerases present, other than in archaebacteria, only in plants. Here we report the isolation of full-length cDNAs encoding putative TOP6 subunits A and B from rice (Oryza sativa ssp. indica), preserving all the structural domains conserved among archaebacterial TOP6 homologs and eukaryotic meiotic recombination factor SPO11. OsTOP6A1 was predominantly expressed in prepollinated flowers. The transcript abundance of OsTOP6A2  ...[more]

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