Genomics

Dataset Information

298

Genome wide localization of chromatin bound NUP98-PHF23 (NP23)


ABSTRACT: We analyzed the genome wide localization of H3K4me3, H3K27me3 and the NUP98-PHF23 (with V5 tag) fusion protein which binds H3K4me3 via its PHD finger, using ChIP-seq. Results correlated with gene expression profiles. NUP98-PHF23 bound only 1.6% of H3K4me3 marks including Hoxa/b + Meis1. Assess H3K4me3 and H3K27me3 histone marks, and correlate these marks with chromatin binding of the NP23 fusion protein using lymphoblast and myeloblast cell lines derived from NP23 leukemias.

ORGANISM(S): Mus musculus  

SUBMITTER: Marbin Pineda   Sheryl M Gough  Robert L Walker  Sven Bilke  Peter D Aplan  Paul S Meltzer  Yuelin Jack Zhu  Fan Yang  Yuelin Zhu 

PROVIDER: E-GEOD-54786 | ArrayExpress | 2014-03-06

SECONDARY ACCESSION(S): GSE54786SRP036859PRJNA237651

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

NUP98-PHF23 is a chromatin-modifying oncoprotein that causes a wide array of leukemias sensitive to inhibition of PHD histone reader function.

Gough Sheryl M SM   Lee Fan F   Yang Fan F   Walker Robert L RL   Zhu Yeulin J YJ   Pineda Marbin M   Onozawa Masahiro M   Chung Yang Jo YJ   Bilke Sven S   Wagner Elise K EK   Denu John M JM   Ning Yi Y   Xu Bowen B   Wang Gang Greg GG   Meltzer Paul S PS   Aplan Peter D PD  

Cancer discovery 20140217 5


In this report, we show that expression of a NUP98-PHF23 (NP23) fusion, associated with acute myeloid leukemia (AML) in humans, leads to myeloid, erythroid, T-cell, and B-cell leukemia in mice. The leukemic and preleukemic tissues display a stem cell-like expression signature, including Hoxa, Hoxb, and Meis1 genes. The PHF23 plant homeodomain (PHD) motif is known to bind to H3K4me3 residues, and chromatin immunoprecipitation experiments demonstrated that the NP23 protein binds to chromatin at a  ...[more]

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